Feline Infectious Peritonitis Ag Test

Feline Infectious Peritonitis Ag Test

INTENDED USE

Feline Infectious Peritonitis Virus Ag Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of FIP-triggerred Coronavirus antigen(FCoV Ag) in cat’s feces, serum and ascites.

Assay Time: 15 - 20 min

Sample: Mixture of serum and feces extraction, ascites or secretions

PRINCIPLE OF THE ASSAY

Feline Infectious Peritonitis Virus Ag Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough FCoV antigen in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Feline Infectious Peritonitis triggerred Coronavirus in the sample.

KIT COMPONENT

• 10×foil pouches, each containing a cassette, pipette and desiccant
• 10×assay buffer tubes (1.0 mL each)
• 10×centrifugal tubes
• 10×swab sticks
• Product Manual

TEST PROCEDURE

• Collect samples seperately from fresh feline feces (liquid part), serum, and cats ascites (if possible) into the diluents tube. Shake the tube sufficiently to mix them well.
• Take out the cassette from the foil pouch and place it horizontally.
• Gradually drip 3 drops of sample extraction into the sample hole.
• Interpret the result in 15 - 20 minutes. Result after 20 minutes is only considered as reference.

INTERPRETATION OF RESULTS

Positive: The presence of both C band and T band, no matter T band is clear or vague.

Negative: Only clear C band appears.

Invalid: No colored band appears in C zone, no matter whether T band appears.

STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

PRECAUTIONS

• For best results, please strictly adhere to these instructions.
• All reagents must be at room temperature before running the assay.
• Do not remove test cassette from its pouch until immediately before use.
• Do not reuse the test kit.
• Do not use the test beyond its expiration date marked on the foil pouch.
• The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

LIMITATION

Feline Infectious Peritonitis Ag Test is only an auxiliary tool for determining the FIP disease. It is used to detect the FIP-triggerred Coronavirus and can only be considered as an in vitro rapid diagnostic reference for veterinarians in practice. FIP is a complex immunity disorder disease and is very difficult to be detected out by only one method. Vets should also consider about the clinical symptoms in practice and results from other diagnostic tools to give a comprehensive judgement.

Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10^th and 11^th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.

Our mission:

• Always best of all and always pay attention to innovation.
• Special customized service tightly following customers’ requests.
• Integrated excellent quality, competitive prices and super service together.

Rebecca Yan